hel92 1 7 cells Search Results


sphk 2 sc 22704  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sphk 2 sc 22704
Abundance of sphingosine kinase <t>(SphK)-1/SphK-2</t> mRNA/protein and sphingosine 1-phosphate (S1P) content of 3T3-L1 cells during adipogenesis. Shown are the results of experiments in which mouse 3T3-L1 cells underwent a treatment protocol with a hormonal stimulation to induce adipogenesis. Cells were treated with the adipogenic stimuli for the times indicated in each panel as described in detail under Materials and Methods. They were then harvested and subjected to either RNA isolation followed by real-time quantitative RT-PCR (qRT-PCR) (A), protein isolation followed by immunoblot analyses (B), or lipid isolation followed by HPLC analyses (C). In panel A, reverse-transcribed template cDNA samples were subjected to qRT-PCR using the primers specific to mouse SphK-1, SphK-2, as well as 18S. Each data point represents the fold increase in transcript expression levels of SphK-1 (closed circles) and SphK-2 (opened circles) relative to those of 18S, normalized to the values obtained from the cells before hormonal stimulation. n = 5. Panel B shows representative images of immunoblots probed for SphK-1, SphK-2, and Rac1 proteins, derived from five independent experiments that yielded equivalent results. Panel C shows the results of S1P measurement using HPLC. Each data point represents mean ± SEM of pooled data derived from three to eight independent experiments. * P < 0.01, ** P < 0.001 vs. values obtained at the time point 0 in each panel.
Sphk 2 Sc 22704, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sphk 2 sc 22704 - by Bioz Stars, 2026-06
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hel92.1.7  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures hel92.1.7
Abundance of sphingosine kinase <t>(SphK)-1/SphK-2</t> mRNA/protein and sphingosine 1-phosphate (S1P) content of 3T3-L1 cells during adipogenesis. Shown are the results of experiments in which mouse 3T3-L1 cells underwent a treatment protocol with a hormonal stimulation to induce adipogenesis. Cells were treated with the adipogenic stimuli for the times indicated in each panel as described in detail under Materials and Methods. They were then harvested and subjected to either RNA isolation followed by real-time quantitative RT-PCR (qRT-PCR) (A), protein isolation followed by immunoblot analyses (B), or lipid isolation followed by HPLC analyses (C). In panel A, reverse-transcribed template cDNA samples were subjected to qRT-PCR using the primers specific to mouse SphK-1, SphK-2, as well as 18S. Each data point represents the fold increase in transcript expression levels of SphK-1 (closed circles) and SphK-2 (opened circles) relative to those of 18S, normalized to the values obtained from the cells before hormonal stimulation. n = 5. Panel B shows representative images of immunoblots probed for SphK-1, SphK-2, and Rac1 proteins, derived from five independent experiments that yielded equivalent results. Panel C shows the results of S1P measurement using HPLC. Each data point represents mean ± SEM of pooled data derived from three to eight independent experiments. * P < 0.01, ** P < 0.001 vs. values obtained at the time point 0 in each panel.
Hel92.1.7, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hel92.1.7/product/European Collection of Authenticated Cell Cultures
Average 90 stars, based on 1 article reviews
hel92.1.7 - by Bioz Stars, 2026-06
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hel92.1.7  (JCRB Cell Bank)
90
JCRB Cell Bank hel92.1.7
Abundance of sphingosine kinase <t>(SphK)-1/SphK-2</t> mRNA/protein and sphingosine 1-phosphate (S1P) content of 3T3-L1 cells during adipogenesis. Shown are the results of experiments in which mouse 3T3-L1 cells underwent a treatment protocol with a hormonal stimulation to induce adipogenesis. Cells were treated with the adipogenic stimuli for the times indicated in each panel as described in detail under Materials and Methods. They were then harvested and subjected to either RNA isolation followed by real-time quantitative RT-PCR (qRT-PCR) (A), protein isolation followed by immunoblot analyses (B), or lipid isolation followed by HPLC analyses (C). In panel A, reverse-transcribed template cDNA samples were subjected to qRT-PCR using the primers specific to mouse SphK-1, SphK-2, as well as 18S. Each data point represents the fold increase in transcript expression levels of SphK-1 (closed circles) and SphK-2 (opened circles) relative to those of 18S, normalized to the values obtained from the cells before hormonal stimulation. n = 5. Panel B shows representative images of immunoblots probed for SphK-1, SphK-2, and Rac1 proteins, derived from five independent experiments that yielded equivalent results. Panel C shows the results of S1P measurement using HPLC. Each data point represents mean ± SEM of pooled data derived from three to eight independent experiments. * P < 0.01, ** P < 0.001 vs. values obtained at the time point 0 in each panel.
Hel92.1.7, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hel92.1.7/product/JCRB Cell Bank
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erythroleukemia hel 92.1.7 (hel) cells  (BioResource International Inc)
90
BioResource International Inc erythroleukemia hel 92.1.7 (hel) cells
Abundance of sphingosine kinase <t>(SphK)-1/SphK-2</t> mRNA/protein and sphingosine 1-phosphate (S1P) content of 3T3-L1 cells during adipogenesis. Shown are the results of experiments in which mouse 3T3-L1 cells underwent a treatment protocol with a hormonal stimulation to induce adipogenesis. Cells were treated with the adipogenic stimuli for the times indicated in each panel as described in detail under Materials and Methods. They were then harvested and subjected to either RNA isolation followed by real-time quantitative RT-PCR (qRT-PCR) (A), protein isolation followed by immunoblot analyses (B), or lipid isolation followed by HPLC analyses (C). In panel A, reverse-transcribed template cDNA samples were subjected to qRT-PCR using the primers specific to mouse SphK-1, SphK-2, as well as 18S. Each data point represents the fold increase in transcript expression levels of SphK-1 (closed circles) and SphK-2 (opened circles) relative to those of 18S, normalized to the values obtained from the cells before hormonal stimulation. n = 5. Panel B shows representative images of immunoblots probed for SphK-1, SphK-2, and Rac1 proteins, derived from five independent experiments that yielded equivalent results. Panel C shows the results of S1P measurement using HPLC. Each data point represents mean ± SEM of pooled data derived from three to eight independent experiments. * P < 0.01, ** P < 0.001 vs. values obtained at the time point 0 in each panel.
Erythroleukemia Hel 92.1.7 (Hel) Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erythroleukemia hel 92.1.7 (hel) cells/product/BioResource International Inc
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erythroleukemia hel 92.1.7 (hel) cells - by Bioz Stars, 2026-06
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Image Search Results


Abundance of sphingosine kinase (SphK)-1/SphK-2 mRNA/protein and sphingosine 1-phosphate (S1P) content of 3T3-L1 cells during adipogenesis. Shown are the results of experiments in which mouse 3T3-L1 cells underwent a treatment protocol with a hormonal stimulation to induce adipogenesis. Cells were treated with the adipogenic stimuli for the times indicated in each panel as described in detail under Materials and Methods. They were then harvested and subjected to either RNA isolation followed by real-time quantitative RT-PCR (qRT-PCR) (A), protein isolation followed by immunoblot analyses (B), or lipid isolation followed by HPLC analyses (C). In panel A, reverse-transcribed template cDNA samples were subjected to qRT-PCR using the primers specific to mouse SphK-1, SphK-2, as well as 18S. Each data point represents the fold increase in transcript expression levels of SphK-1 (closed circles) and SphK-2 (opened circles) relative to those of 18S, normalized to the values obtained from the cells before hormonal stimulation. n = 5. Panel B shows representative images of immunoblots probed for SphK-1, SphK-2, and Rac1 proteins, derived from five independent experiments that yielded equivalent results. Panel C shows the results of S1P measurement using HPLC. Each data point represents mean ± SEM of pooled data derived from three to eight independent experiments. * P < 0.01, ** P < 0.001 vs. values obtained at the time point 0 in each panel.

Journal: Journal of Lipid Research

Article Title: Sphingosine kinase is induced in mouse 3T3-L1 cells and promotes adipogenesis s⃞

doi: 10.1194/jlr.M800206-JLR200

Figure Lengend Snippet: Abundance of sphingosine kinase (SphK)-1/SphK-2 mRNA/protein and sphingosine 1-phosphate (S1P) content of 3T3-L1 cells during adipogenesis. Shown are the results of experiments in which mouse 3T3-L1 cells underwent a treatment protocol with a hormonal stimulation to induce adipogenesis. Cells were treated with the adipogenic stimuli for the times indicated in each panel as described in detail under Materials and Methods. They were then harvested and subjected to either RNA isolation followed by real-time quantitative RT-PCR (qRT-PCR) (A), protein isolation followed by immunoblot analyses (B), or lipid isolation followed by HPLC analyses (C). In panel A, reverse-transcribed template cDNA samples were subjected to qRT-PCR using the primers specific to mouse SphK-1, SphK-2, as well as 18S. Each data point represents the fold increase in transcript expression levels of SphK-1 (closed circles) and SphK-2 (opened circles) relative to those of 18S, normalized to the values obtained from the cells before hormonal stimulation. n = 5. Panel B shows representative images of immunoblots probed for SphK-1, SphK-2, and Rac1 proteins, derived from five independent experiments that yielded equivalent results. Panel C shows the results of S1P measurement using HPLC. Each data point represents mean ± SEM of pooled data derived from three to eight independent experiments. * P < 0.01, ** P < 0.001 vs. values obtained at the time point 0 in each panel.

Article Snippet: Primary antibodies used were those raised against SphK-1 (PC727) (Calbiochem, San Diego, CA) and SphK-2 (sc-22704) (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Isolation, Quantitative RT-PCR, Western Blot, Expressing, Derivative Assay

Effects of small interfering RNA (siRNA) targeted to mouse SphK-1 on SphK-1 and SphK-2 protein expression in 3T3-L1 cells. The effects of siRNA targeted to mouse SphK-1 mRNA on protein expression levels of 3T3-L1 cells were examined in immunoblot analyses. 3T3-L1 cells were transiently transfected with siRNA specific to SphK-1 or control 40 h prior to hormonal stimulation. Five days after hormonal stimulation, cells were lysed and resulting lysates were subjected to immunoblot analyses using an antibody specific to SphK-1, SphK-2, and Rac1. Upper panels indicate representative images of immunoblots, derived from six independent experiments. The lower panel shows the results of densitometric analyses from pooled data. Protein abundance of SphK-1 as well as that of SphK-2 was normalized by that of Rac1 in control- and SphK-1-specific siRNA groups, respectively. The bars indicate percent changes obtained in cells treated with SphK-1-specific siRNA relative to those with control siRNA. ** P < 0.01 vs. values obtained with control siRNA.

Journal: Journal of Lipid Research

Article Title: Sphingosine kinase is induced in mouse 3T3-L1 cells and promotes adipogenesis s⃞

doi: 10.1194/jlr.M800206-JLR200

Figure Lengend Snippet: Effects of small interfering RNA (siRNA) targeted to mouse SphK-1 on SphK-1 and SphK-2 protein expression in 3T3-L1 cells. The effects of siRNA targeted to mouse SphK-1 mRNA on protein expression levels of 3T3-L1 cells were examined in immunoblot analyses. 3T3-L1 cells were transiently transfected with siRNA specific to SphK-1 or control 40 h prior to hormonal stimulation. Five days after hormonal stimulation, cells were lysed and resulting lysates were subjected to immunoblot analyses using an antibody specific to SphK-1, SphK-2, and Rac1. Upper panels indicate representative images of immunoblots, derived from six independent experiments. The lower panel shows the results of densitometric analyses from pooled data. Protein abundance of SphK-1 as well as that of SphK-2 was normalized by that of Rac1 in control- and SphK-1-specific siRNA groups, respectively. The bars indicate percent changes obtained in cells treated with SphK-1-specific siRNA relative to those with control siRNA. ** P < 0.01 vs. values obtained with control siRNA.

Article Snippet: Primary antibodies used were those raised against SphK-1 (PC727) (Calbiochem, San Diego, CA) and SphK-2 (sc-22704) (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Small Interfering RNA, Expressing, Western Blot, Transfection, Derivative Assay

Expression of SphK-1 and SphK-2 mRNA in adipose tissue of ob/ob mouse. Shown are the results of qRT-PCR analyses using RNA samples from obese mice or their littermates. RNA was extracted from subcutaneous adipose tissue and spleen of 13-week-old male mice of either ob/ob or WT genetic background. RNA samples were subjected to qRT-PCR assays as above using primer pairs directed to SphK-1, SphK-2, and 18S. Transcript abundance of SphK-1 as well as SphK-2 in each tissue sample was normalized to that of 18S. Values obtained from ob/ob mouse tissue was normalized to those obtained in WT samples, and are shown as relative abundance of SphK-1 (upper panel) and SphK-2 (lower panel). Each data point represents mean ± SEM of pooled data, derived from five independent animals in each genetic group.

Journal: Journal of Lipid Research

Article Title: Sphingosine kinase is induced in mouse 3T3-L1 cells and promotes adipogenesis s⃞

doi: 10.1194/jlr.M800206-JLR200

Figure Lengend Snippet: Expression of SphK-1 and SphK-2 mRNA in adipose tissue of ob/ob mouse. Shown are the results of qRT-PCR analyses using RNA samples from obese mice or their littermates. RNA was extracted from subcutaneous adipose tissue and spleen of 13-week-old male mice of either ob/ob or WT genetic background. RNA samples were subjected to qRT-PCR assays as above using primer pairs directed to SphK-1, SphK-2, and 18S. Transcript abundance of SphK-1 as well as SphK-2 in each tissue sample was normalized to that of 18S. Values obtained from ob/ob mouse tissue was normalized to those obtained in WT samples, and are shown as relative abundance of SphK-1 (upper panel) and SphK-2 (lower panel). Each data point represents mean ± SEM of pooled data, derived from five independent animals in each genetic group.

Article Snippet: Primary antibodies used were those raised against SphK-1 (PC727) (Calbiochem, San Diego, CA) and SphK-2 (sc-22704) (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Expressing, Quantitative RT-PCR, Derivative Assay